ABSTRACT
Objective: To study the expressions of AAX14401 in the normal bladder tissue and the human bladder transitional cell carcinoma tissue, and to analyze its function in the occurrence and development of bladder cancer. Methods: Seventy-five cases of specimens of bladder transitional cell carcinoma were collected and used as experimental group and 12 cases of normal bladder mucosa specimens were selected as control group. The positive expression rates of glutathione S-transferase pi-1 (GSTP1) and AAX14401 were detected by immunohistochemistry. The differences of positive expression rates of GSTP1 and AAX14401 proteins between bladder cancer tissue and normal bladder tissue were analyzed. Results: The positive expression rate of GSTP1 protein in normal bladder tissue was 58.3%, while it was 90.7% in bladder cancer tissue, and the difference between them was significant (P<0. 01). The positive expression rate of AAX14401 protein in normal bladder tissue was 0%, while it was 2. 7% in blandder cancer tissue, and there was no significant difference between them (P= 0. 596). There was no one with positive AAX14401 expression and positive GSTP1 expression in bladder cancer tissue at the same time. There were 2 cases with positive AAX14401 expression in bladder cancer tissue with negative GSTP1 expression; there was a negative correlation between the positive expressions of AAX14401 and GSTP1 proteins in bladder cancer (r=-0. 274, P=0. 018). Conclusion: The expression of AAX14401 protein may promote the incidence and progression of bladder cancer.
ABSTRACT
Objective To investigate the efficacy of ventral bladder mucosa onlay graft urethroplasty for the management of panurethral stricture.Methods From August of 2005 to July of 2013,11 cases of panurethral stricture were treated by ventral bladder mucosa onlay graft urethroplasty.The median age of the patients was 53 years (22-72 year),The median stricture length was 15 cm (12-18 cm).The patient was placed in the lithotomy position,Penile urethra was exposed by circumcoronal incision and degloving of skin,Bulbar urethra was exposed by inverted Y-shaped perineal incision.The strictured urethral segment was then opened ventrally in the midline up to at least 1 cm proximally into the healthy urethra.An appropriate size bladder mucosa graft was harvested,and was quilted to the splited urethra edge,the graft width was 1.5-2.0 cm.Two F10 fenestrated silicone catheters were left as urethral stents,a suprapupic cystostomy tube was left.The urethral stent was removed 4 weeks postoperatively.Follow-up was performed every 3 months for the first year,and annually thereafter.Success was defined as normal voiding with a maximum flow rates ≥ 15 ml/s,and the patients required no further instrumentation,including dilation or urethrotomy.Results The mean follow-up was 18 months (range,9-36 months),the overall success rate was 10/11.One patient developed urethral meatus stenosis 3 months postoperatively,and was managed by meatal dilatation.Conclusion Ventral bladder mucosa onlay graft urethroplasty can be used for the management of panurethral stricture,Bladder mucosa is an alternative substitution for complex urethral reconstruction.